ECOS™ 21 BL21(DE3) Competent Cells FYE207-40VL

Description: This strain provides high levels of protein expression. This strain carries the lambda DE3 lysogen, which expresses T7 RNA polymerase from the lacUV5 promoter by isopropyl-1-thio-ß-D-galactopyranoside (IPTG) induction. The transformation efficiency of BL-21 is usually low, so the 6 minute/ heat shock cold plating protocol is recommended if high efficiency is desired. Genotype F– ompT gal dcm lon hsdSB(rB– mB–) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) Features: lack of Lon and Omp proteases allows for high-level protein expression and accumulation, Easy gene induction. Applications: IPTG induced gene expression in E. coli, Ideal for high-level expression of non-toxic recombinant protein expression using T7-based expression vectors & Subcloning