Product info:

The heterodimer with SLC3A2 functions as sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, L-DOPA, leucine, histidine, methionine and tryptophan. Functions as an amino acid exchanger. May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity. May act as the major transporter of tyrosine in fibroblasts (Probable. May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity. Can mediate the transport of thyroid hormones triiodothyronine (T3 and thyroxine (T4 across the cell membrane. When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation. Involved in the uptake of toxic methylmercury (MeHg when administered as the L-cysteine or D,L-homocysteine complexes. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO across the membrane.

Alternative names:

SLC7A5; 4F2LC; CD98; D16S469E; E16; LAT1; MPE16; hLAT1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Apical cell membrane, Cytoplasm, Multi-pass membrane protein, cytosol

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SLC7A5 (NP_003477.4).

Positive control:

HT-1080

AA Sequence:

MAGAGPKRRALAAPAAEEKEEAREKMLAAKSADGSAPAGEGEGVTLQRNITLLNGVAIIVGTIIGSGIFVTPTGVLKEAGSPGLALVVWAACGVFSIVGALCYAELGTTISKSGGDYAYMLEVYG

Purification:

Affinity purification

Molecular weight:

Calculated MW: 55kDa Observed MW: 45KDa

Form:

Liquid

Applications:

WB (Sus scrofa)

Dilution:

WB 1:500 - 1:1000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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