Product info:

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Alternative names:

TOP1; TOPI

Species reactivity:

Human, Mouse

Host:

Rabbit

Cellular localisation:

Nucleus, nucleolus, nucleoplasm

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1).

Positive control:

MCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary, Mouse testis

AA Sequence:

MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA

Purification:

Affinity purification

Molecular weight:

Calculated MW: 90kDa Observed MW: 108kDa

Form:

Liquid

Applications:

WB (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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