Product info:

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported.

Alternative names:

B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200; PTPRC

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Membrane, Membrane raft, Single-pass type I membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 890-990 of human CD45 (NP_002829.2).

Positive control:

Jurkat

AA Sequence:

RCLMVQVEAQYILIHQALVEYNQFGETEVNLSELHPYLHNMKKRDPPSEPSPLEAEFQRLPSYRSWRTQHIGNQEENKSKNRNSNVIPYDYNRVPLKHELE

Purification:

Affinity purification

Molecular weight:

Calculated MW: 130kDa/147kDa Observed MW: 180-250KDa

Form:

Liquid

Applications:

IHC (Human chronic myeloid leukemia cells, Mus musculus) IF (Human chronic myeloid leukemia cells, Mus musculus) WB (Mus musculus) FC (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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