Product info:

This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative names:

GLI1; GLI

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus, Nucleus

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human GLI1 (NP_001153517.1).

Positive control:

Mouse brain

AA Sequence:

EKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESR

Purification:

Affinity purification

Molecular weight:

Calculated MW: 104kDa/113kDa/117kDa Observed MW: 150KDa

Form:

Liquid

Applications:

WB (Homo sapiens, Rattus norvegicus) IHC (Homo sapiens, Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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