Product info:

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17.

Alternative names:

SREBF1; SREBP-1c; SREBP1; SREBP1a; bHLHd1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

COPII-coated vesicle membrane, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein, Multi-pass membrane protein, Nucleus

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human SREBF1 (NP_001005291.1).

Positive control:

Mouse brain, Rat brain

AA Sequence:

MDEPPFSEAALEQALGEPCDLDAALLTDIEGEVGAGRGRANGLDAPRAGADRGAMDCTFEDMLQLINNQDSDFPGLFDPPYAGSGAGGTDPASPDTSSPG

Purification:

Affinity purification

Molecular weight:

Calculated MW: 45kDa/48kDa/111kDa/113kDa/121kDa/124kDa Observed MW: 125KDa

Form:

Liquid

Applications:

WB (Mus musculus, Gallus gallus, Sus scrofa)

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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