Product info:

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants.

Alternative names:

IL6; BSF-2; BSF2; CDF; HGF; HSF; IFN-beta-2; IFNB2; IL-6

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Secreted

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 30-212 of human IL6 (NP_000591.1).

Positive control:

Rat lung

AA Sequence:

VPPGEDSKDVAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLEFEVYLEYLQNRFESSEEQARAVQMSTKVLIQFLQKKAKNLDAITTPDPTTNASLLTKLQAQNQWLQDMTTHLILRSFKEFLQSSLRALRQM

Purification:

Affinity purification

Molecular weight:

Calculated MW: 23kDa Observed MW: 26KDa

Form:

Liquid

Applications:

WB (Mouse embryonal fibroblast cell, Human colon cancer cell, Mouse skeletal muscle, Mus musculus, Rattus norvegicus, Homo sapiens, Oryctolagus cuniculus, Cynoglossus semilaevis) IF (Human colon cancer cell, Homo sapiens, Mus musculus) IHC (Mus musculus, Homo sapiens, Rattus norvegicus) ELISA (Mus musculus)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com