Product info:

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.

Alternative names:

TLR4; ARMD10; CD284; TLR-4; TOLL

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell membrane, Single-pass type I membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human TLR4 (NP_612564.1).

Positive control:

HL-60, RAW264.7

AA Sequence:

FLDLSQCQLEQLSPTAFNSLSSLQVLNMSHNNFFSLDTFPYKCLNSLQVLDYSLNHIMTSKKQELQHFPSSLAFLNLTQNDFACTCEHQSFLQWIKDQRQL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 73kDa/91kDa/95kDa Observed MW: 98KDa

Form:

Liquid

Applications:

WB (Raw264.7, Rat spinal cord, Mouse cells, BV2, Mus musculus, Rattus norvegicus, Homo sapiens, Sus scrofa) IHC (Mouse kidney, Rattus norvegicus, Mus musculus) IF (Mus musculus) FC (Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

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