Product info:

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants.

Alternative names:

TJP1; ZO-1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell junction, Cell membrane, Cytoplasmic side, Peripheral membrane protein, gap junction, tight junction

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 1600-1700 of human ZO-1 (NP_003248.3).

Positive control:

LO2, HeLa, NIH/3T3, Mouse uterus, Rat liver

AA Sequence:

PKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMC

Purification:

Affinity purification

Molecular weight:

Calculated MW: 186kDa/195kDa Observed MW: 250kDa

Form:

Liquid

Applications:

WB (Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Sus scrofa) IF (Mus musculus, Rattus norvegicus, Homo sapiens)

Dilution:

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

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