Product info:

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2, thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-628 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2. Plays an important role in the regulation of DNA replication by stabilizing the licensing factor CDT1.

Alternative names:

USP37

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

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Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 150-250 of human USP37 (NP_065986.2).

Positive control:

U-87MG, HeLa, DU145, Mouse spleen, Rat testis

AA Sequence:

SLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 99kDa/110kDa Observed MW: 100kDa

Form:

Liquid

Applications:

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Dilution:

WB 1:500 - 1:2000

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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