Product info:

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Alternative names:

TNF; DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F; TNF alpha

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cell membrane, Membrane, Secreted, Single-pass type II membrane protein, Single-pass type II membrane protein

Isotype:

IgG

Immunogen:

A synthetic peptide of human TNF

Positive control:

THP-1, RAW264.7, PC-12

AA Sequence:

Email info@sobekbio.com for sequence

Purification:

Affinity purification

Molecular weight:

Calculated MW: 25kDa Observed MW: 28KDa

Form:

Liquid

Applications:

IHC (Mouse breast cancer, Homo sapiens, Mus musculus, Rattus norvegicus) ChIP (Homo sapiens, Mus musculus) IF (Rattus norvegicus, Homo sapiens, Mus musculus) WB (Homo sapiens, quails, Mus musculus, Rattus norvegicus, Gallus gallus)

Dilution:

WB 1:1000 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com