Product info:

Prostaglandin-endoperoxide synthase (PTGS), also known as cyclooxygenase, is the key enzyme in prostaglandin biosynthesis, and acts both as a dioxygenase and as a peroxidase. There are two isozymes of PTGS: a constitutive PTGS1 and an inducible PTGS2, which differ in their regulation of expression and tissue distribution. This gene encodes the inducible isozyme. It is regulated by specific stimulatory events, suggesting that it is responsible for the prostanoid biosynthesis involved in inflammation and mitogenesis.

Alternative names:

PTGS2; COX-2; COX2; GRIPGHS; PGG/HS; PGHS-2; PHS-2; hCox-2; Cox2

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein

Isotype:

IgG

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 375-604 of human COX2/PTGS2 (NP_000954.1).

Positive control:

A-549, THP-1, RAW264.7

AA Sequence:

PLLPDTFQIHDQKYNYQQFIYNNSILLEHGITQFVESFTRQIAGRVAGGRNVPPAVQKVSQASIDQSRQMKYQSFNEYRKRFMLKPYESFEELTGEKEMSAELEALYGDIDAVELYPALLVEKPRPDAIFGETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL

Purification:

Affinity purification

Molecular weight:

Calculated MW: 68kDa Observed MW: 74KDa

Form:

Liquid

Applications:

WB (Mouse embryonal fibroblast cell, RAW264.7, Mouse kidney, Mus musculus, Rattus norvegicus, Trifolium pratense, Schisandra chinensis, Homo sapiens, Gallus gallus) IHC (RAW264.7, Mouse kidney, Mus musculus, Rattus norvegicus) IF (Mus musculus, Homo sapiens)

Dilution:

WB 1:1000 - 1:4000 IHC 1:50 - 1:200 IF 1:50 - 1:100

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com