Product info:

This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid.

Alternative names:

NLRP3; AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Endoplasmic reticulum, Inflammasome, Nucleus, Secreted, cytosol

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human NLRP3 (NP_001120934.1).

Positive control:

Raji, RAW264.7

AA Sequence:

CCWDLSTLLTSSQSLRKLSLGNNDLGDLGVMMFCEVLKQQSCLLQNLGLSEMYFNYETKSALETLQEEKPELTVVFEPSW

Purification:

Affinity purification

Molecular weight:

Calculated MW: 83kDa/105kDa/111kDa/112kDa/115kDa/118kDa Observed MW: 105KDa

Form:

Liquid

Applications:

IF (Mouse colon cancer cells, Mus musculus, Gallus gallus, Rattus norvegicus) WB (Mouse colon cancer cells, Mus musculus, Rattus norvegicus, Gallus gallus, Homo sapiens) IHC (Mus musculus, Rattus norvegicus)

Dilution:

WB 1:1000 - 1:3000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

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