Product info:

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Alternative names:

CCNB; CCNB1; Cyclin B1; cyclin B1

Species reactivity:

Human, Mouse, Rat

Host:

Rabbit

Cellular localisation:

Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center

Isotype:

IgG

Immunogen:

A synthetic peptide corresponding to a sequence within amino acids 330 to the C-terminus of human Cyclin B1 (NP_114172.1).

Positive control:

C2C12, C6, Mouse liver, Rat testis

AA Sequence:

TMLDYDMVHFPPSQIAAGAFCLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV

Purification:

Affinity purification

Molecular weight:

Calculated MW: 44kDa/48kDa Observed MW: 55KDa

Form:

Liquid

Applications:

WB (H460, Human tumor cell, MEF, A172, HeLa, HCT116, Homo sapiens, Mus musculus, Rattus norvegicus)

Dilution:

WB 1:500 - 1:2000 IF 1:50 - 1:200

Storage buffer:

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Storage:

Store at 4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com