Product overview:

VHL (Von Hippel Lindau) mouse monoclonal antibody, clone LBI1E1

Field of research:

Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Summary:

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Alternative names:

HRCA1; pVHL; RCA1; VHL1

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

VHL

Isotype:

IgG2b

Immunogen:

Full length human recombinant protein of human VHL(NP_000542) produced in HEK293T cell.

Clone:

LBI1E1

Conjugation:

Unconjugated

Gene name:

von Hippel-Lindau tumor suppressor

Symbol:

VHL

Pathway:

Pathways in cancer, Renal cell carcinoma, Ubiquitin mediated proteolysis

Function:

Druggable Genome, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:4000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com