Product overview:

PHF7 mouse monoclonal antibody, clone LBI1A11

Field of research:

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Summary:

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. In the testis, this gene is expressed in Sertoli cells but not germ cells. However, this gene is not expressed in a patient who exhibited spermatogenic arrest at the spermatocyte stage. Spermatogenic arrest is an interruption of germ cell differentiation that may result in oligospermia or azoospermia. The proteins encoded by this gene contain plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. Thus this protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Two protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq, Jul 2008]

Alternative names:

HSPC045; HSPC226; NYD-SP6

Species reactivity:

Human

Host:

Mouse

Target:

PHF7

Isotype:

IgG1

Immunogen:

Human recombinant protein fragment corresponding to amino acids 155-381 of human PHF7(NP_057567) produced in E.coli.

Clone:

LBI1A11

Conjugation:

Unconjugated

Gene name:

PHD finger protein 7

Symbol:

PHF7

Pathway:

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Function:

Druggable Genome, Transcription Factors

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

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Orders:

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