Product overview:

RFC2 mouse monoclonal antibody, clone LBI3F6

Field of research:

DNA replication, Mismatch repair, Nucleotide excision repair

Summary:

The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins, proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also called activator 1, is a protein complex consisting of five distinct subunits of 145, 40, 38, 37, and 36.5 kD. This gene encodes the 40 kD subunit, which has been shown to be responsible for binding ATP. Deletion of this gene has been associated with Williams syndrome. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Alternative names:

RFC40

Species reactivity:

Human, Mouse, Rat

Host:

Mouse

Target:

RFC2

Isotype:

IgG1

Immunogen:

Full length human recombinant protein of huamn RFC2(NP_002905) produced in E.coli.

Clone:

LBI3F6

Conjugation:

Unconjugated

Gene name:

replication factor C subunit 2

Symbol:

RFC2

Pathway:

DNA replication, Mismatch repair, Nucleotide excision repair

Function:

Druggable Genome, Stem cell - Pluripotency

Purification:

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

Concentration:

1 mg/ml

Form:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Applications & dilution:

WB 1:2000, IHC 1:150

Storage buffer:

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Storage:

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com