Background:

Catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA(dsRNA) referred to as A-to-I RNA editing. This may affect gene expression and function in a number of ways that include mRNA translation by changing codons and hence the amino acid sequence of proteins; pre-mRNA splicing by altering splice site recognition sequences; RNA stability by changing sequences involved in nuclease recognition; genetic stability in the case of RNA virus genomes by changing sequences during viral RNA replication; and RNA structure-dependent activities such as microRNA production or targeting or protein-RNA interactions. Can edit both viral and cellular RNAs and can edit RNAs at multiple sites(hyper-editing) or at specific sites(site-specific editing). Its cellular RNA substrates include: bladder cancer-associated protein(BLCAP), neurotransmitter receptors for glutamate(GRIA2 and GRIK2) and serotonin(HTR2C), GABA receptor(GABRA3) and potassium voltage-gated channel(KCNA1). Site-specific RNA editing of transcripts encoding these proteins results in amino acid substitutions which consequently alter their functional activities. Edits GRIA2 at both the Q/R and R/G sites efficiently but converts the adenosine in hotspot1 much less efficiently. Can exert a proviral effect towards human immunodeficiency virus type 1(HIV-1) and enhances its replication via both an editing-dependent and editing-independent mechanism. The former involves editing of adenosines in the 5'UTR while the latter occurs via suppression of EIF2AK2/PKR activation and function. Can inhibit cell proliferation and migration and can stimulate exocytosis.

Clonality:

polyclonal

Classification:

Primary antibody

Alternative names:

ADAR2, ADAR2a, ADAR2a L1, ADAR2a L2, ADAR2a L3, ADAR2b, ADAR2c, ADAR2d, ADAR2g, ADARB1, DRABA2, DRADA2, dsRNA adenosine deaminase, RED1, RNA editing deaminase 1, RNA editing enzyme 1

Specificity:

Human, Rat

Host:

Rabbit

Isotype:

IgG

Immunogen:

adenosine deaminase, RNA-specific, B1(RED1 homolog rat)

Purification:

Immunogen affinity purified

Purity:

≥95% as determined by SDS-PAGE

Calculated molecular weight:

81 kDa

Form:

liquid

Applications:

ELISA, WB

Recommended dilution:

WB: 1:500-1:5000; IP: 1:500-1:5000

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com