Alternative names:

CMT1F;CMT2E;CMTDIG;NF-L;NF68;NFL;PPP1R110

Description:

Recombinant Human NEFL(9-88) with C-terminal human Fc tag

Background:

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

Molecular characterization:

NEFL(Thr9-Thr88) hFc(Glu99-Ala330)

Molecular weight:

The protein has a predicted molecular mass of 34.9 kDa after removal of the signal peptide. The apparent molecular mass of NEFL(9-88)-hFc is approximately 35-55 kDa due to glycosylation.

Tag:

C-Human Fc Tag

Expression host:

HEK293

Target:

NEFL

Purity:

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation / Reconstitution:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage:

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

More info:

Email info@sobekbio.com

Orders:

Email orders@sobekbio.com