Alternative names:

C23;Nsr1

Description:

Recombinant human NCL protein with N-terminal 6×His tag

Background:

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Molecular characterization:

6×His tag NCL(Val2-Glu710)

Molecular weight:

The protein has a predicted molecular mass of 77.3 kDa after removal of the signal peptide. The apparent molecular mass of His-NCL is approximately 100-130 kDa due to glycosylation.

Tag:

N-6×His Tag

Expression host:

HEK293

Target:

NCL

Purity:

The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.

Formulation / Reconstitution:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage:

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

More info:

Email info@sobekbio.com

Orders:

Email orders@sobekbio.com