Alternative names:

D12S53E;gp100;ME20;ME20-M;ME20M;P1;P100;PMEL17;SI;SIL;SILV

Description:

Recombinant human PMEL Protein with C-terminal Human Fc tag

Background:

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Molecular characterization:

PMEL(Lys25-Ala595) hFc(Glu99-Ala330)

Molecular weight:

The protein has a predicted molecular mass of 46.2 kDa after removal of the signal peptide.The apparent molecular mass of PMEL-hFc is approximately 130-250 kDa due to glycosylation.

Tag:

C-Human Fc Tag

Expression host:

HEK293

Target:

PMEL

Purity:

The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Formulation / Reconstitution:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Storage:

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

More info:

Email info@sobekbio.com

Orders:

Email orders@sobekbio.com