Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Type I(IL1R1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Receptor Type I(IL1R1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Type I(IL1R1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Type I(IL1R1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;CD & Adhesion molecule;Infection immunity;

Species reactivity:

Mouse

Alternative names:

IL-1R1;CD121a; CD121-A; IL1-R1; IL1R; IL1RA; P80; CD121 Antigen-Like Family Member A; Interleukin-1 Receptor Alpha

Sample type:

Serum, plasma, tissue homogenates and other biological fluids.

Detection range:

31.25-2000 pg/mL

Sensitivity:

14.8 pg/mL

Standard:

2000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com