Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 1(CCL1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 1(CCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Ligand 1(CCL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Ligand 1(CCL1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Cytokine;Tumor immunity;Infection immunity;

Species reactivity:

Mouse

Alternative names:

SCYA1; I-309; TCA3; P500; SISe; Small Inducible Cytokine A1; T Lymphocyte Secreted Protein I-309

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

3.2 pg/mL

Standard:

500 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com