Product info:

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Fibroblast Growth Factor 23(FGF23) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Fibroblast Growth Factor 23(FGF23). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Fibroblast Growth Factor 23(FGF23) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Cytokine;Tumor immunity;Infection immunity;Genetic science;

Species reactivity:

Rat

Alternative names:

ADHR; HYPF; HPDR2; PHPTC; Phosphatonin; Tumor-derived hypophosphatemia-inducing factor

Sample type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

0.92 pg/mL

Standard:

200 pg/mL

Detection method:

Competitive Inhibition

Assay time:

2h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com