Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Susceptibility Protein 1(BRCA1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Breast Cancer Susceptibility Protein 1(BRCA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Susceptibility Protein 1(BRCA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Breast Cancer Susceptibility Protein 1(BRCA1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Tumor immunity;

Species reactivity:

Human

Alternative names:

BRCAI; BRCC1; IRIS; PSCP; RNF53; Breast Cancer 1,Early Onset; RING finger protein 53

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.065 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com