Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,4-Galactosyltransferase 1(b4GALT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Beta-1,4-Galactosyltransferase 1(b4GALT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,4-Galactosyltransferase 1(b4GALT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1,4-Galactosyltransferase 1(b4GALT1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Enzyme & Kinase;Metabolic pathway;

Species reactivity:

Human

Alternative names:

GTB; B4-GALT1; GGTB2; GT1; Beta4Gal-T1; Lactose Synthase A Protein; N-Acetyllactosamine Synthase; Nal synthase; beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Sample type:

Serum, plasma, tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.59 ng/mL

Standard:

100 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com