Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Neutral Amino Acid Transporter 1(LAT1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Large Neutral Amino Acid Transporter 1(LAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Neutral Amino Acid Transporter 1(LAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Neutral Amino Acid Transporter 1(LAT1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Metabolic pathway;Infection immunity;

Species reactivity:

Human

Alternative names:

CD98; MDU1; 4T2HC; 4F2; NACAE; SLC7A5; MPE16; Solute Carrier Family 7 Member 5; Cationic Amino Acid Transporter,y+ System; L-Type Amino Acid Transporter

Sample type:

tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.058 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com