Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CUB Domain Containing Protein 1(CDCP1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CUB Domain Containing Protein 1(CDCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CUB Domain Containing Protein 1(CDCP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CUB Domain Containing Protein 1(CDCP1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;Tumor immunity;

Species reactivity:

Human

Alternative names:

CD318; CDCP1; SIMA135; TRASK; Membrane glycoprotein gp140; Transmembrane and associated with src kinases; Subtractive immunization M plus HEp3-associated 135 kDa

Sample type:

Tissue homogenates, cell lysates and other biological fluids.

Detection range:

Sensitivity:

0.124 ng/mL

Standard:

20 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com