Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elastase 3B(ELA3B). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Elastase 3B(ELA3B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elastase 3B(ELA3B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Elastase 3B(ELA3B) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Enzyme & Kinase;

Species reactivity:

Human

Alternative names:

CELA3B; Chymotrypsin Like Elastase Family Member 3B; Protease E; Fecal Elastase; Elastase 1; Cholesterol-Binding Pancreatic Protease; Pancreatic Endopeptidase E

Sample type:

Serum, plasma and other biological fluids.

Detection range:

Sensitivity:

0.135 ng/mL

Standard:

20 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com