Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 1(NHE1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 1(NHE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 1(NHE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 1(NHE1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Human

Alternative names:

SLC9A1; APNH; Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger; Antiporter,Na+/H+,Amiloride Sensitive; Na(+)/H(+) antiporter, amiloride-sensitive

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.054 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com