At Solulink, we develop high-performance products that link biomolecules together. Our continuous efforts in new product development and service offerings enable you to remain at the forefront of advanced bioscience research. The Company’s proprietary HyNic/4FB chemistry is unique among bioconjugation methods in combining superb stability with high specificity and versatility, suitable for conjugation of all classes of biomolecules, including peptides, proteins, oligonucleotides, and carbohydrates, with all types of labels, including fluorophores, chromogenic moieties, surfaces, and small molecules. HyNic/4FB chemistry underlies the majority of Solulink’s extensive product catalog, comprised of various kits and tools that enable efficient and stable antibody, bead, and oligonucleotide labeling.
Human CSF2Rb(Colony Stimulating Factor 2 Receptor Beta) ELISA Kit ELK4301
Specifications
| 48T / 96T |
Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2 Receptor Beta(CSF2Rb). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Colony Stimulating Factor 2 Receptor Beta(CSF2Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor 2 Receptor Beta(CSF2Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Colony Stimulating Factor 2 Receptor Beta(CSF2Rb) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Signal transduction;CD & Adhesion molecule;
Species reactivity:
Human
Alternative names:
CD131; CSF2R-B; IL3-RB; IL3RB; CSF2-RB; Colony Stimulating Factor 2 Receptor,Beta,Low-Affinity(granulocyte-Macrophage); GM-CSF/IL-3/IL-5 receptor common beta subunit
Sample type:
tissue homogenates, cell lysates and other biological fluids
Detection range:
Sensitivity:
0.113 ng/mL
Standard:
20 ng/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com