At Solulink, we develop high-performance products that link biomolecules together. Our continuous efforts in new product development and service offerings enable you to remain at the forefront of advanced bioscience research. The Company’s proprietary HyNic/4FB chemistry is unique among bioconjugation methods in combining superb stability with high specificity and versatility, suitable for conjugation of all classes of biomolecules, including peptides, proteins, oligonucleotides, and carbohydrates, with all types of labels, including fluorophores, chromogenic moieties, surfaces, and small molecules. HyNic/4FB chemistry underlies the majority of Solulink’s extensive product catalog, comprised of various kits and tools that enable efficient and stable antibody, bead, and oligonucleotide labeling.
Human HSD11b2(11-Beta-Hydroxysteroid Dehydrogenase Type 2) ELISA Kit ELK4346
Specifications
| 48T / 96T |
Product info:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2(HSD11b2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2(HSD11b2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2(HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2(HSD11b2) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Enzyme & Kinase;
Species reactivity:
Human
Alternative names:
AME; AME1; HSD11K; HSD2; SDR9C3; Short Chain Dehydrogenase/Reductase Family 9C,Member 3; NAD-dependent 11-beta-hydroxysteroid dehydrogenase
Sample type:
Tissue homogenates, cell lysates and other biological fluids.
Detection range:
Sensitivity:
33 pg/mL
Standard:
5000 pg/mL
Detection method:
Sandwich
Assay time:
3.5h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com