Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proline/Serine Rich Coiled Coil protein 1(PSRC1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Proline/Serine Rich Coiled Coil protein 1(PSRC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Proline/Serine Rich Coiled Coil protein 1(PSRC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Proline/Serine Rich Coiled Coil protein 1(PSRC1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Human

Alternative names:

DDA3; Differential Display And Activated By p53

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

Sensitivity:

0.048 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com