General 5-HETE(5-Hydroxyeicosatetraenoic Acid) ELISA Kit ELK8155
Specifications
| 48T / 96T |
Product info:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 5-Hydroxyeicosatetraenoic Acid(5-HETE) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5-Hydroxyeicosatetraenoic Acid(5-HETE). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxyeicosatetraenoic Acid(5-HETE) in the samples is then determined by comparing the OD of the samples to the standard curve.
Research area:
Enzyme & Kinase;
Species reactivity:
General
Alternative names:
5-HETE
Sample type:
Serum, plasma and other biological fluids
Detection range:
Sensitivity:
3.95 ng/mL
Standard:
800 ng/mL
Detection method:
Competitive Inhibition
Assay time:
2h
More info:
Email: info@sobekbio.com
Orders:
Email: info@sobekbio.com

ImmuSmol is specialized in developing antibodies directed against small molecules. Supported by public institutions including BPI, Conseil Régional Nouvelle Aquitaine, as well as by its Co-founder Associate – SCHIEVER, Immusmol is established in Bordeaux, France.