Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Autophagy Related Protein 7(ATG7). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Autophagy Related Protein 7(ATG7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Autophagy Related Protein 7(ATG7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Autophagy Related Protein 7(ATG7) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Metabolic pathway;

Species reactivity:

Rat

Alternative names:

APG7L; GSA7; Ubiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Ubiquitin-activating enzyme E1-like protein

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.055 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com