Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PDZK1IP1(PDZK1-interacting protein 1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PDZK1IP1(PDZK1-interacting protein 1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PDZK1IP1(PDZK1-interacting protein 1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PDZK1IP1(PDZK1-interacting protein 1) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Cancer

Species reactivity:

Mouse

Alternative names:

MAP17; 17 kDa membrane associated protein; DD96; Epithelial protein up regulated in carcinoma membrane associated protein 17; Membrane associated protein 17; PDZK1-interacting protein 1; Pdzk1ip1; RP1-18D14.5; SPAP;

Sample type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection range:

Sensitivity:

13.2 pg/mL

Standard:

2000 pg/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com