Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 3A5(CYP3A5). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cytochrome P450 3A5(CYP3A5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 3A5(CYP3A5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 3A5(CYP3A5) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Tumor immunity;

Species reactivity:

Mouse

Alternative names:

CP35; P450PCN3; PCN3; Cytochrome P450,Subfamily IIIA(Niphedipine Oxidase),Polypeptide 5; Cytochrome P450 HLp2

Sample type:

Tissue homogenates, cell lysates and other biological fluids

Detection range:

Sensitivity:

0.59 ng/mL

Standard:

100 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com