Product info:

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphoinositide-3-Kinase Class-2-Alpha Polypeptide(PIK3C2a) in the samples is then determined by comparing the OD of the samples to the standard curve.

Research area:

Signal transduction;

Species reactivity:

Human

Alternative names:

PI3K2a; CPK; PI3-K-C2(Alpha); PI3-K-C2A; PI3K2-a; Phosphoinositide 3-Kinase 2a; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha

Sample type:

Tissue homogenates and other biological fluids.

Detection range:

0.16-10 ng/mL

Sensitivity:

0.057 ng/mL

Standard:

10 ng/mL

Detection method:

Sandwich

Assay time:

3.5h

More info:

Email: info@sobekbio.com

Orders:

Email: info@sobekbio.com