Hot Start Taq DNA Polymerase is an enzyme solution in which a monoclonal antibody against Taq enzyme and Taq enzyme are mixed in a 1:1 activity unit. Taq enzyme antibody has a very high affinity with Taq enzyme. It can block the activity of Taq enzyme before high temperature denaturation, so it can inhibit primer dimer and non-specific amplification very effectively, greatly improving the accuracy of PCR reaction. Hot Start Taq DNA Polymerase does not require special high temperature treatment. The pre-denaturation step in conventional PCR reaction conditions can start the activity of Taq enzyme. It is suitable for various Taq enzyme-based hot-start PCR and qPCR reactions. The PCR product has an A at the 3'end, which can be cloned directly with TA vector.