Description:

DiTagTM pH sensitive IgG labeling reagent

Background:

DiTagTM pH sensitive IgG labeling reagents provide an easy solution to measure internalization activities of antibodies. This reagent utilizes a pH-sensitive fluorescently labeled Fc binding protein that binds to IgG antibodies from various species, resulting in the formation of a fluorescently labeled antibody-reagent complex. After antibody internalization, the surrounding pH becomes acidic and significantly enhances fluorescence signal of antibody-reagent complex. The fluorescence intensity can be used as an indicator to determine the internalization activity of antibodies. By measuring the strength of the fluorescence signal, researchers can assess the efficiency of antibody internalization into cells. This information is crucial in understanding the cellular uptake mechanism of antibodies and assessing their efficacy in targeted therapies or diagnostic applications. Additionally, monitoring the fluorescence intensity can also provide insights into the kinetics of antibody internalization, helping researchers optimize experimental condition and improve the design of antibody-based drug delivery systems.

Applications:

Antibody internalization labeling kit

Detection method:

Flow cytometry, detected with FITC or AF488 filter

Excitation / Emission:

505/525 nm

Molecular weight:

The product has a MW of 33.4 kDa

IgG type:

The DiTagTM pH sensitive IgG labeling reagents can be used for human IgG1, IgG2 and IgG4, rabbit IgG, mouse IgG2a and IgG2b.

Formulation / Reconstitution:

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.

Recommended dilution:

We recommend test antibody to mix with AME100001 at 2:1 in molar

Storage:

The reagents are supplied in lyophilized form. We recommend storing the vial(s) at -20℃, desiccated and protected from light. Once reconstituted, the reagents can be stored at 2-8℃ for 1~2 weeks, or with 50% glycerol at -20℃.

Usage:

Research use only

More info:

Email info@sobekbio.com

Orders:

Email orders@sobekbio.com