Background:

Based on immunoelectrophoresis and/or ELISA, the antibody reacts with whole molecule sheep IgG.It also reacts with the light chains of other sheeep immunoglobuins. No antibody was detected against non-immunoglobulin serum proteins. The antibody exhibits inherent minimal cross-reaction to mouse serum proteins and has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with mouse,human,and rabbit serum proteins. The antibody may cross-react with immunoglobulins from other species.

Description:

Peroxidase-conjugated AffiniPure Rabbit Anti-Sheep IgG (H+L)

Classification:

Secondary Antibodies

Host:

Rabbit

Immunogen:

Sheep IgG (H+L)

Label:

Peroxidase(HRP)

Purification & Purity:

The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Concentration:

0.8 mg/ml

Applications:

WB ELISA IHC ICC

Suggested dilution range:

1:500 -1:5000 for immunohisto/cytochemistry 1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000-1:200,000 for Western blotting with ECL substrates

Buffer:

0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6,containing 1% BSA,50% glycerol

Preservative:

0.05% Sodium Azide

Storage:

Store at 4°C. Avoid freeze-thaw cycles. Prepare working dileuion fresh each day.

Expiry:

One year from date of reconstitution.

More info:

Email: info@sobekbio.com

Orders:

Email: orders@sobekbio.com