Host:

E.coli

Tag:

His-tag

AA Sequence:

GAAAGCCGCGCCGAACTGACCAGCGATAAAGATATGTATCTGGATAATAGCAGCATTGAAGAAGCAAGCGGTGTGTATCCGATTGATGATGATGATTATGCAAGTGCCAGCGGTAGCGGTGCAGATGAAGATGTGGAAAGCCCGGAACTGACCACCAGTCGTCCGCTGCCGAAAATTCTGCTGACCAGCGCCGCCCCGAAAGTTGAAACCACCACCCTGAATATTCAGAATAAAATTCCGGCCCAGACCAAAAGTCCGGAAGAAACCGATAAAGAAAAAGTGCATCTGAGCGATAGTGAACGCAAAATGGATCCGGCAGAAGAAGATACCAATGTGTATACCGAAAAACATAGCGATAGTCTGTTCAAACGCACCGAA

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~27kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Syndecans are type I integral membrane proteoglycans that contain both chondroitin sulfate and heparan sulfate groups. Syndecans are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 (SYND1, also called CD138) is anextracellular matrix receptor which binds to collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 (also designated N-Syndecan) interact with MK (midkine), a growth/differentiation factor invloved in embryogenesis of the central nervous system. Syndecan-2 (also designated fibroglycan or HSPG) is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 (also designated amphiglycan or ryudocan) functions cooperativley with integrins in the processes of cell spreading, focal adhesion assembly and Actin stress fiber assembly

Note:

For research use only, not for use in diagnostic procedure.