Host:

E.coli

Tag:

His-tag

AA Sequence:

CAGAGTAAAGCCCAGGTTCTGCAGAGCGTGGCCGGTCAGACCCTGACCGTGCGTTGTCAGTATCCGCCGACCGGCAGCCTGTATGAAAAAAAAGGTTGGTGTAAAGAAGCAAGTGCACTGGTGTGTATTCGTCTGGTGACCAGCAGTAAACCGCGTACCATGGCATGGACCAGTCGCTTCACCATCTGGGATGATCCGGATGCCGGCTTCTTCACCGTTACCATGACCGATCTGCGCGAAGAAGATAGTGGCCATTATTGGTGCCGTATCTATCGTCCGAGTGATAATAGCGTGAGCAAAAGCGTTCGCTTCTATCTGGTTGTTAGTCCGGCAAGTGCAAGCACCCAGACCAGCTGGACCCCGCGTGATCTGGTTAGTAGCCAGACCCAGACCCAGAGTTGCGTGCCGCCGACCGCCGGTGCACGTCAAGCTCCTGAAAGTCCGAGCACCATTCCGGTTCCGAGTCAGCCGCAGAATAGTACCCTGCGTCCGGGCCCGGCAGCACCTATTGCA

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~19kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Natural killer (NK) cells direct cytotoxicity against tumor or virally infected cells. NK cell-mediated cytotoxicity is stimulated by several activating receptors associated with the signaling adapter DNAX activation 12/killer cellactivating receptor-associated protein (DAP12). NKp44 is a natural cytotoxicity receptor that is expressed on IL-2-activated human NK cells and may contribute to the increased efficiency of NK cells to mediate tumor cell lysis. NKp44 is composed of one Ig-like extracellular domain, a transmembrane segment and a cytoplasmic domain. Prolactin upregulates and cortisol downregulates the surface expression of NKp44 at the transcriptional level. A cellular ligand for NKp44 (NKp44L) is expressed during HIV-1 infection and is correlated with the progression of CD4+ T cell depletion and an increase of viral load. This implicates NKp44 as a therapeutic agent that may aid in the progress towards a vaccine for HIV-1 infection.

Note:

For research use only, not for use in diagnostic procedure.