Host:

E.coli

Tag:

His-tag

AA Sequence:

AAGGCACAGGTTAATCCGGCAATCTGTCGCTATCCGCTGGGCATGAGTGGTGGTCAGATTCCGGATGAAGATATTACCGCCAGCAGCCAGTGGAGTGAAAGTACCGCCGCCAAATATGGCCGCCTGGATAGCGAAGAAGGTGATGGTGCCTGGTGTCCGGAAATTCCGGTGGAACCGGATGATCTGAAAGAATTCCTGCAGATTGATCTGCATACCCTGCACTTCATTACCCTGGTTGGCACCCAGGGTCGCCATGCAGGCGGTCATGGTATTGAATTCGCACCGATGTATAAAATTAACTATAGCCGCGATGGCACCCGTTGGATTAGTTGGCGTAATCGCCATGGCAAACAGGTGCTGGATGGCAATAGCAATCCGTATGATATCTTCCTGAAAGATCTGGAACCGCCGATTGTGGCCCGCTTCGTGCGCTTCATTCCGGTGACCGATCATAGTATGAATGTGTGTATGCGCGTGGAACTGTATGGTTGTGTGTGGCTGGATGGTCTGGTGAGCTATAATGCACCGGCCGGCCAGCAGTTCGTTCTGCCGGGTGGTAGCATTATCTATCTGAATGATAGTGTGTATGACGGCGCCGTTGGTTATAGTATGACCGAAGGTCTGGGTCAGCTGACCGATGGTGTTAGCGGTCTGGATGACTTCACCCAGACCCATGAATATCATGTGTGGCCGGGTTATGATTATGTTGGCTGGCGCAATGAAAGTGCAACCAATGGCTATATTGAAATTATGTTCGAATTCGATCGCATTCGTAACTTCACCACCATGAAAGTGCATTGCAATAATATGTTCGCCAAAGGCGTGAAAATCTTCAAAGAAGTGCAGTGCTACTTCCGCAGTGAAGCCAGTGAATGGGAACCGAATGCCATTAGCTTCCCGCTGGTGCTGGATGATGTTAATCCGAGCGCCCGCTTCGTTACCGTGCCGCTGCATCATCGTATGGCAAGCGCAATTAAATGCCAGTATCACTTCGCAGATACCTGGATGATGTTCAGCGAAATTACCTTCCAGAGTGATGCAGCAATGTATAATAATAGTGAAGCACTGCCGACCAGTCCGATGGCCCCGACCACCTATGATCCGATGCTGAAAGTTGATGATAGTAATACCCGT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~48kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2. They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling. Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion. Injection of colon tumor cells into DDR2 knock out mice predisposes the hepatic stellate cells to colon tumor metastasis.

Note:

For research use only, not for use in diagnostic procedure.