Host:

E.coli

Tag:

His-tag

AA Sequence:

CTGATGGGTGGCCAGGATAAACCGTTCCTGAGTGCACGCCCGAGTACCGTGGTTCCGCGCGGTGGTCATGTGGCACTGCAGTGCCATTATCGCCGCGGCTTCAATAACTTCATGCTGTATAAAGAAGACCGTAGCCATGTGCCGATCTTCCATGGTCGTATCTTCCAGGAAAGCTTCATTATGGGTCCGGTTACCCCGGCACATGCCGGCACCTATCGTTGCCGCGGTAGTCGTCCGCATAGTCTGACCGGCTGGAGCGCCCCGAGCAATCCGCTGGTGATTATGGTTACCGGCAATCATCGTAAACCGAGTCTGCTGGCCCATCCGGGTCCGCTGCTGAAAAGTGGCGAAACCGTTATTCTGCAGTGCTGGAGCGATGTGATGTTCGAACACTTCTTCCTGCATCGCGAAGGTATTAGTGAAGATCCGAGCCGCCTGGTGGGTCAGATTCATGATGGTGTTAGCAAAGCCAACTTCAGTATTGGTCCGCTGATGCCGGTGCTGGCAGGTACCTATCGTTGTTATGGTAGCGTTCCGCATAGTCCGTATCAGCTGAGTGCACCGAGTGATCCGCTGGATATTGTTATTACCGGCCTGTATGAAAAACCGAGTCTTAGTGCACAGCCGGGCCCGACCGTGCAGGCAGGTGAAAATGTGACCCTGAGTTGTAGTAGCTGGAGCAGCTATGATATCTATCATCTGAGCCGCGAAGGCGAAGCCCATGAACGCCGTCTGCGCGCCGTTCCGAAAGTGAATCGTACCTTCCAGGCCGACTTCCCGCTGGGTCCGGCCACACATGGTGGCACCTATCGCTGCTTCGGTAGCTTCCGTGCCCTGCCGTGTGTGTGGAGCAATAGCAGTGATCCGTTACTGGTTAGCGTGACCGGCAATCCGAGCAGCAGCTGGCCGAGTCCGACCGAACCGAGTAGCAAAAGCGGTATCTGTCGTCATCTGCAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~35kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

The killer immunoglobulin-like receptors (KIRs) on natural killer (NK) cells regulate the inhibition and activation of NK-cell responses through recognition of human leukocyte antigen (HLA) class I molecules. KIR3DL1, a receptor for HLA-B antigens with the Bw4 allele, transmits an inhibitory signal to prevent killer cell-mediated cytoxicity. KIR3DL1 encodes a 444 amino acid type I transmembrane protein, containing 3 immunoglobulin-like C2-type domains. Human KIR3DL1 maps to chromosome 19q13.4.

Note:

For research use only, not for use in diagnostic procedure.