Host:

E.coli

Tag:

His-tag

AA Sequence:

TGGCCTCCTCCGGGTACCGGTGATGTTGTGGTTCAGGCACCGACCCAGGTGCCGGGCTTCCTGGGTGATAGTGTTACCCTGCCGTGTTATCTGCAGGTTCCGAATATGGAAGTGACCCATGTTAGTCAGCTGACCTGGGCACGCCATGGTGAAAGCGGTAGCATGGCCGTGTTCCATCAGACCCAGGGTCCGAGTTATAGCGAAAGCAAACGCCTGGAATTCGTGGCAGCCCGCCTGGGTGCAGAACTGCGTAATGCCAGTCTGCGCATGTTCGGCCTGCGCGTGGAAGATGAAGGCAATTATACCTGCCTGTTCGTTACCTTCCCGCAGGGTAGTCGTAGCGTGGATATCTGGCTGCGCGTGCTGGCCAAACCGCAGAATACCGCCGAAGTTCAGAAAGTGCAGCTGACCGGTGAACCGGTGCCGATGGCACGCTGTGTGAGCACCGGTGGTCGTCCGCCGGCACAGATTACCTGGCATAGTGATCTGGGTGGTATGCCGAATACCAGCCAGGTGCCGGGCTTCTTAAGTGGTACCGTGACCGTGACCAGTCTGTGGATTCTGGTGCCGAGCAGTCAGGTTGATGGCAAAAATGTTACCTGTAAAGTTGAACATGAGAGCTTCGAAAAACCGCAGCTGCTGACCGTGAATCTGACCGTGTATTATCCGCCGGAAGTTAGCATTAGCGGCTATGATAATAATTGGTATCTGGGCCAGAATGAAGCCACCCTGACCTGCGATGCCCGCAGTAATCCGGAACCGACCGGTTATAATTGGAGTACCACCATGGGCCCGCTGCCGCCGTTCGCAGTGGCACAAGGTGCACAGCTGCTGATTCGCCCGGTGGATAAACCGATTAATACCACCCTGATCTGTAATGTTACCAATGCACTGGGCGCACGTCAGGCCGAACTGACCGTTCAGGTTAAAGAAGGTCCGCCGAGTGAACATAGTGGTATTAGCCGTAAT

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~36kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Poliovirus receptor (PVR, CD155) is an immunoglobulin-like, transmembrane glycoprotein originally described as a mediator of poliovirus attachment to cells and later identified as important in adherens junction formation. Also known as nectin-like 5 (Necl-5), PVR binds nectin-3 and interacts with integrin αvβ3 and PDGFR to regulate integrin clustering and focal contact formation at the leading edge of migrating cells. Research studies demonstrate that PVR and nectin-3 regulate contact inhibition during cell motility and proliferation in transformed 3T3 cells. Additional research indicates that PVR (CD155, Necl-5) expression may play a role in invasiveness of lung adenocarcinoma. In the immune system, CD155 plays a role in natural killer (NK) cell-mediated cytotoxicity.

Note:

For research use only, not for use in diagnostic procedure.