Host:

E.coli

Tag:

His-tag

AA Sequence:

GATTGGATCTGGAATCAGATGCATATTGATGAAGAAAAAAACACCAGTCTGCCGCATCATGTGGGTAAAATTAAAAGCAGTGTGAGCCGCAAAAATGCAAAATATCTGCTGAAAGGTGAATATGTGGGTAAAGTGTTCCGCGTTGATGCAGAAACCGGTGATGTGTTCGCAATTGAACGTCTGGATCGTGAAAATATTAGTGAATATCATCTGACCGCAGTGATTGTTGATAAAGATACCGGTGAAAATCTGGAAACACCTAGTAGCTTCACCATTAAAGTGCATGATGTGAATGATAATTGGCCGGTGTTCACCCATCGTCTGTTCAATGCAAGCGTTCCGGAAAGTAGCGCCGTGGGTACCAGTGTTATTAGCGTTACCGCCGTTGATGCAGATGATCCGACCGTTGGTGATCATGCAAGTGTTATGTATCAGATTCTGAAAGGCAAAGAATACTTCGCCATTGATAATAGTGGTCGCATTATTACCATTACCAAAAGTCTGGATCGCGAAAAACAGGCCCGCTATGAAATTGTTGTGGAAGCACGTGATGCACAGGGCCTGCGCGGCGATAGTGGTACCGCAACCGTTCTGGTGACCCTGCAGGATATTAATGATAACTTCCCGTTCTTCACCCAGACCAAATATACCTTCGTGGTTCCGGAAGATACCCGCGTGGGCACCAGCGTGGGTAGCCTGTTCGTTGAAGATCCGGATGAACCGCAGAATCGTATGACCAAATATAGTATTCTGCGCGGTGATTATCAGGATGCCTTCACCATTGAAACCAATCCGGCACATAATGAAGGCATTATTAAACCGATGAAACCGCTGGATTATGAATATATTCAGCAGTATAGCTTCATCGTGGAAGCAACCGATCCGACCATTGATCTGCGCTATATGAGTCCGCCGGCCGGTAATCGTGCACAGGTTATTATTAATATTACCGATGTTGACGAGCCGCCGATCTTCCAGCAGCCGTTCTATCACTTCCAGCTGAAAGAAAATCAGAAAAAACCGCTGATTGGCACCGTTCTGGCCATGGATCCGGATGCAGCCCGTCATAGCATTGGTTATAGTATTCGTCGCACCAGCGATAAAGGTCAGTTCTTCCGCGTGACCAAAAAAGGCGATATCTATAATGAAAAGGAGCTGGATCGCGAGGTGTATCCGTGGTATAATCTGACCGTTGAAGCAAAAGAACTGGATAGTACCGGCACCCCGACCGGCAAAGAAAGCATTGTTCAGGTTCATATTGAAGTGCTGGATGAAAATGATAATGCACCGGAATTCGCCAAACCGTATCAGCCGAAAGTGTGTGAAAATGCAGTGCATGGTCAGCTGGTGCTGCAGATTAGCGCCATTGATAAAGATATTACCCCGCGTAATGTGAAATTCAAATTCATTCTGAACACCGAAAACAACTTCACCCTGACCGATAATCATGATAATACCGCAAATATTACCGTGAAATATGGCCAGTTCGATCGCGAACATACCAAAGTTCACTTCCTGCCGGTGGTGATTAGTGATAATGGCATGCCGAGCCGTACCGGCACCAGTACCCTGACCGTTGCCGTGTGCAAATGTAATGAACAGGGTGAATTCACCTTCTGTGAAGATATGGCAGCCCAGGTGGGCGTTAGTATTCAG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~61kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development. The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton. While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking. Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers. Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis. Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers.

Note:

For research use only, not for use in diagnostic procedure.