Host:

E.coli

Tag:

His-tag

AA Sequence:

CTGGATCCGGGTCTGCAGCCGGGTAACTTCAGTGCAGATGAAGCAGGTGCACAGCTGTTCGCACAGAGCTATAATAGTAGCGCAGAACAGGTTCTGTTCCAGAGCGTTGCCGCAAGTTGGGCCCATGATACCAATATTACCGCCGAAAATGCCCGTCGCCAGGAAGAAGCCGCCCTGCTGAGTCAGGAATTCGCAGAAGCCTGGGGCCAGAAAGCAAAAGAACTGTATGAACCGATCTGGCAGAACTTCACCGATCCGCAGCTGCGTCGCATTATTGGTGCAGTGCGTACCCTGGGTAGCGCAAATCTGCCGCTGGCAAAACGTCAGCAGTATAATGCACTGCTGAGCAATATGAGTCGTATCTATAGTACCGCAAAAGTGTGCCTGCCGAATAAAACCGCAACCTGTTGGAGTCTGGATCCGGATCTGACCAATATTCTGGCAAGTAGCCGCAGTTATGCCATGCTGCTGTTCGCCTGGGAAGGTTGGCATAATGCAGCCGGCATTCCGCTGAAACCGCTGTATGAAGACTTCACCGCACTGAGCAATGAAGCATATAAACAGGATGGCTTCACCGATACCGGTGCATATTGGCGTAGTTGGTATAATAGCCCGACCTTCGAAGATGATCTGGAACATCTGTATCAGCAGCTGGAACCGCTGTATCTGAATCTGCATGCATTCGTGCGTCGCGCCCTGCATCGCCGTTATGGTGATCGTTATATTAATCTGCGCGGTCCGATTCCGGCACATCTGCTGGGCGATATGTGGGCCCAGAGCTGGGAAAATATCTATGATATGGTGGTGCCGTTCCCGGATAAACCGAATCTGGATGTGACCAGTACCATGCTGCAGCAGGGTTGGAATGCCACCCACATGTTCCGTGTTGCAGAAGAATTCTTCACCAGCCTGGAACTGAGTCCGATGCCGCCGGAATTCTGGGAAGGTAGCATGCTGGAAAAACCGGCCGATGGTCGTGAAGTTGTGTGCCATGCAAGTGCATGGGACTTCTATAATCGCAAAGACTTCCGCATTAAACAGTGTACCCGCGTGACCATGGATCAGCTGAGCACCGTGCATCATGAAATGGGCCATATTCAGTATTATCTGCAGTATAAAGACCTGCCGGTGAGCCTGCGCCGTGGTGCAAATCCGGGCTTCCATGAAGCCATTGGCGATGTTCTGGCACTGAGCGTTAGCACCCCGGAACATCTGCATAAAATTGGTCTGCTGGATCGTGTGACCAATGATACCGAAAGCGATATTAATTATCTGCTGAAAATGGCACTGGAAAAAATTGCATTCCTGCCGTTCGGTTATCTGGTTGATCAGTGGCGCTGGGGTGTGTTCAGCGGTCGTACCCCGCCGAGCCGTTATAACTTCGATTGGTGGTATCTGCGTACCAAATATCAGGGTATCTGTCCGCCGGTTACCCGCAATGAAACACACTTCGATGCCGGTGCCAAATTCCATGTTCCGAATGTGACCCCGTATATTCGTTACTTCGTGAGCTTCGTGCTGCAGTTCCAGTTCCATGAAGCACTGTGCAAAGAAGCCGGCTATGAAGGTCCGCTGCATCAGTGTGATATCTATCGTAGTACCAAAGCCGGTGCAAAACTGCGTAAAGTGCTGCAGGCCGGCAGTAGCCGTCCGTGGCAGGAAGTTCTGAAAGATATGGTGGGTCTGGATGCACTGGATGCCCAGCCGCTGCTGAAATACTTCCAGCCGGTTACCCAGTGGCTGCAGGAACAGAATCAGCAGAATGGCGAAGTTCTGGGCTGGCCGGAATATCAGTGGCATCCG

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~66kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exopeptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Although it bears significant sequence homology to ACE, ACE2 shows a more restricted pattern of expression. ACE is expressed ubiquitously throughout the vasculature while ACE2 is expressed only in cardiac, renal and testicular cells.

Note:

For research use only, not for use in diagnostic procedure.