Host:

E.coli

Tag:

His-tag

AA Sequence:

GCACCTGCAGAACCGCAGCCGGGTGGCAGTCAGTGTGTGGAACATGATTGCTTCGCCCTGTATCCGGGCCCGGCAACCTTCCTGAATGCAAGTCAGATCTGTGATGGTCTGCGTGGCCATCTGATGACCGTTCGTAGTAGTGTGGCCGCCGATGTGATTAGTCTGCTGCTGAATGGTGATGGCGGTGTTGGCCGTCGTCGCCTGTGGATTGGCCTGCAGCTGCCGCCGGGCTGTGGCGACCCTAAACGTCTGGGCCCGCTGCGTGGCTTCCAGTGGGTGACCGGCGATAATAATACCAGCTATAGCCGCTGGGCACGCCTGGATCTGAATGGCGCCCCGCTGTGTGGCCCGCTGTGCGTGGCAGTGAGCGCCGCAGAAGCCACCGTGCCGAGTGAACCGATCTGGGAAGAACAGCAGTGTGAAGTTAAAGCAGATGGCTTCCTGTGCGAATTCCACTTCCCGGCAACCTGTCGCCCGCTGGCAGTTGAACCGGGTGCCGCAGCCGCCGCTGTTAGTATTACCTATGGCACCCCGTTCGCCGCCCGTGGTGCCGACTTCCAGGCCTTACCGGTGGGTAGCAGCGCCGCAGTGGCACCTCTGGGCCTGCAGTTAATGTGTACCGCACCGCCGGGTGCCGTTCAGGGTCATTGGGCCCGTGAAGCACCGGGCGCATGGGATTGTAGTGTTGAAAATGGTGGTTGCGAACATGCATGCAATGCAATTCCGGGTGCCCCGCGCTGTCAGTGCCCGGCAGGTGCAGCTCTGCAGGCCGATGGCCGTAGTTGCACCGCAAGCGCCACCCAGAGCTGCAATGATCTGTGTGAACACTTCTGTGTGCCGAATCCGGATCAGCCGGGTAGCTATAGTTGTATGTGCGAAACCGGTTATCGTCTGGCCGCAGATCAGCATCGCTGCGAAGATGTTGATGATTGTATTCTGGAACCGAGCCCGTGTCCGCAGCGTTGCGTTAATACCCAGGGCGGCTTCGAATGCCATTGCTATCCGAATTATGATCTGGTGGATGGTGAATGCGTTGAACCGGTGGATCCGTGCTTCCGTGCAAATTGCGAATATCAGTGTCAGCCGCTGAATCAGACCAGCTATCTGTGCGTGTGTGCCGAAGGCTTCGCACCGATTCCGCATGAACCGCATCGCTGCCAGATGTTCTGCAATCAGACCGCCTGTCCGGCAGATTGCGATCCGAATACCCAGGCAAGCTGCGAATGCCCGGAAGGCTATATTCTGGATGATGGCTTCATCTGTACCGATATTGATGAATGTGAAAATGGCGGCTTCTGCAGCGGCGTGTGTCATAATCTGCCGGGTACCTTCGAATGTATCTGTGGCCCGGATAGCGCACTGGCACGCCATATTGGTACCGATTGTGATAGTGGTAAAGTGGATGGTGGTGATAGTGGCAGCGGTGAACCGCCGCCGAGCCCTACACCGGGTAGTACCCTGACCCCGCCGGCAGTGGGTCTGGTTCATAGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~66kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

CD141/Thrombomodulin (TM, THBD, BDCA-3) is an integral membrane protein expressed on the surface of endothelial cells. Acting as a cofactor with Thrombin, CD141/Thrombomodulin activates and initiates the Protein C anticoagulant pathway. CD141/Thrombomodulin is expressed by a small subset of human CD11c+ myeloid dendritic cells. These CD141+XCR1+ dendritic cells cross-present antigens to naïve CD8+ T cells, priming them to become activated cytotoxic CD8+ T cells.

Note:

For research use only, not for use in diagnostic procedure.