Host:

E.coli

Tag:

His-tag

AA Sequence:

GAAAAGAGCGATCTGCGTACCGTGGCACCGGCAAGCAGCCTGAATGTTCGCTTCGATAGCCGCACCATGAATCTGAGCTGGGATTGCCAGGAAAATACCACCTTCAGTAAATGCTTCCTGACCGATAAAAAAAATCGTGTTGTGGAACCGCGCCTGAGTAATAATGAATGTAGCTGCACCTTCCGTGAAATCTGTCTGCATGAAGGCGTTACCTTCGAAGTGCATGTGAATACCAGCCAGCGTGGCTTCCAGCAGAAACTGCTGTATCCGAATAGTGGCCGTGAAGGTACCGCCGCACAGAACTTCAGTTGCTTCATCTATAATGCAGATCTGATGAATTGTACCTGGGCACGTGGTCCGACCGCACCGCGTGATGTGCAGTACTTCCTGTATATTCGCAATAGTAAACGCCGCCGCGAAATTCGCTGTCCGTATTATATTCAGGATAGTGGTACCCATGTGGGCTGTCATCTGGATAATCTGAGTGGTCTGACCAGTCGCAATTACTTCCTGGTTAATGGTACCAGTCGTGAAATTGGTATTCAGTTCTTCGATAGCCTGCTGGATACCAAAAAAATTGAACGCTTCAATCCGCCGAGTAATGTTACCGTGCGCTGCAATACCACCCATTGCCTGGTTCGTTGGAAACAGCCGCGCACCTATCAGAAACTGAGCTATCTGGACTTCCAGTATCAGCTGGATGTGCATCGTAAAAATACCCAGCCGGGTACCGAAAATCTGCTGATTAATGTTAGCGGTGATCTGGAAAATCGTTATAACTTCCCGAGTAGCGAACCGCGTGCAAAACATAGCGTGAAAATTCGTGCCGCAGATGTGCGTATTCTGAATTGGAGCAGTTGGAGTGAAGCAATTGAATTCGGTAGTGATGATGGC

Expression vector:

pet-22b(+)

Soluble:

PBS, 4M Urea, PH7.4

BiowMW:

~33kDa

Purification & Purity:

Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is > 85% (by SDS-PAGE).

Storage & Stability:

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background:

Granulocyte-macrophage colony-stimulating factor receptor subunit alpha (GM-CSF Receptor α), also known as CSF2RA and CD116, is a type 1 transmembrane protein with low affinity for GM-CSF. GM-CSF Receptor α is primarily expressed on myeloid cells, including granulocytes, monocytes, macrophages, and dendritic cells. GM-CSF Receptor α forms a heterodimeric receptor complex with GM-CSF Receptor β, creating the high affinity GM-CSF receptor. Upon dimerization and ligand binding, the β subunit becomes phosphorylated and transduces signals via Jak/STAT and MAPK pathways for cell proliferation, differentiation, and survival. Oligomerization of the high affinity GM-CSF Receptor α/β complex is required for optimal intracellular signal transduction. Soluble GM-CSF Receptor α, either secreted or cleaved from the cell surface, can competitively bind GM-CSF, preventing membrane receptor signaling. Multiple isoforms of GM-CSF Receptor α have been described.

Note:

For research use only, not for use in diagnostic procedure.